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gennarino.org • Leggi argomento - Ancora lievito madre, la tesi di silvia.


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Ancora lievito madre, la tesi di silvia.

Una Cucina Grande, dove stare in tanti a parlare di tutto.
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Messaggioda sdonk » 12/07/2006, 21:06

Olio extravergine d'oliva pugliese
www.fontanadelcavallo.com
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sdonk
Santo Subito!!!
 
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Messaggioda capsicum » 12/07/2006, 22:47

sdonk, non lo so, francamente. si potrebbe andare per tentativi. Non so come abbiano fatto, ma probabilmetne per i lieviti hanno diluito un grammo di pasta madre in una quantità nota di sol isotonica, probabilmente un cc, poi ne hanno pipettato un ml ed hanno inseminato una piastra. Fai cadere la goccia e strisci con una spatolina passata al bunsen (uguale al cannello per caramellare :eek: ) e adeguatametne raffreddata. Agar con glucosio? boh! potrebbe andar bene, ma anche agar siero, qualcosa di semplice comunque. Poi incubato a quanto? 24 gradi? o 27? La pasta madre lievita a temperatura ambiente, quindi direi tra 24 e 28 gradi centigradi. Poi conti le colonie e le moltiplichi per dieci.
E per i lattacidi non mi ricordo mica come li facevamo, sono passati trent'anni, mica poco. Usavamo tanti di quei terreni che viravano di colore col ph, vatti a ricordare, ma se vuoi contare i produttori di acido lattico probabilmetne ti basta inserire un colorante che vira nel terreno e contare insieme sia le colonie dei lieviti sia quelle dei lattacidi che chiaramente virano il colorante. Butto lì, ma sarebbe tanto più facile chiederlo a un bromatologo, nel laboratori aziendali fanno tutte le analisi sui cibi, direi.
Cecilia Deni
------------
Time is a drug. Too much of it kills you
capsicum
 
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